25% Off Select Antibodies and ELISA Kits
Sign In/Register 0

We use cookies, check our Privacy Policies.

Products
Primary Antibodies
[KO Validated] Beclin 1 Rabbit mAb
[KO Validated] Beclin 1 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0093
Reactivity:Human,Mouse,Rat
Applications:WB,ELISA
Modifcation:Unmodified
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20ul Size Available
#
#
#
#

[KO Validated] Beclin 1 Rabbit mAb

Western blot analysis of lysates from wild type (WT) and Beclin1 knockout (KO) 293T(KO) cells, using [KO Validated] Beclin 1 Rabbit mAb (A22359) at 1:30000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.

Delivery
Contact Us
Telephone
408-747-0185
Fax
408-747-0145
Email
order@assaybiotech.com
Promotions
Product Details
Product Name
[KO Validated] Beclin 1 Rabbit mAb
Description
Beclin 1 plays a central role in autophagy. It acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis.There two casp cleavage sites in Beclin 1, TDVD(133) and DQLD(149), cleavage at which yields fragments lacking the autophagy-inducing capacity. Noteworthy, the C-terminal fragment, Beclin 1-C, localized predominantly at the mitochondria and sensitized the cells to apoptosis. It induces the mitochondrial translocation of BAX and the release of proapoptotic factors.
Catalog No
M0093
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Application Dilutions
WB,1:10000 - 1:30000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Unmodified
Source
Rabbit
Isotype
IgG
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).
Calculated MW (kDa)
0
Observed MW (kDa)Gene Name
60kDaBeclin 1
Other Name
Gene ID
Human Swiss Prot
Formulation
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
Related Products

Catalog No: N1292

β-actin Monoclonal Antibody(5B7)

Price/Quantity:
$184

Size:
100μL

Catalog No: N1293

GAPDH Monoclonal Antibody(2B8)

Price/Quantity:
$184

Size:
100μL

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
    © 2025 AssayBiotech. All Rights Reserved.
    Follow Us
    Terms of Use Privacy Policy